Unknown risk of the reintroduction of malignant cells in a Danish cohort of women autotransplanted with ovarian tissue.

31 03 2011

To the Editor:

Ovarian cryopreservation appears to hold much promise for fertility preservation of women undergoing gonadotoxic therapy. In the Netherlands, cryopreservation of ovarian tissue has been performed for a number of cancer patients. No autotransplantation of ovarian tissue has been performed thus far, due (amongst other reasons) to concerns about the risk of reintroducing the malignancy with the transplant.

The available literature on this subject is not unequivocal. For example, Shaw et al. (1) have shown in a mouse model that lymphoma can be transmitted to the recipient by both fresh and frozen ovarian tissue grafts. Kim et al. (2), on the other hand, reassuringly demonstrated that none of the mice that were xenografted with human ovarian tissue fragments derived from patients with (non)Hodgkin lymphoma developed disease. Read the rest of this entry »





Considerations on: “The role of inositol supplementation in patients with polycystic ovary syndrome, with insulin resistance, undergoing the low-dose gonadotropin ovulation induction regimen”

30 03 2011

To the Editor:

We read with great interest the article by Morgante et al. entitled, “The role of inositol supplementation in patients with polycystic ovary syndrome, with insulin resistance, undergoing the low-dose gonadotropin ovulation induction regimen,” which has been recently published in Fertility and Sterility.

We do have some concerns about methodology and conclusions of this study. Read the rest of this entry »





Suggestion and comment for CC preventing LH surge

30 03 2011

To the Editor:

I read with interest the article by Al-Inany et al. (1) in which the authors demonstrated the beneficial effects of the addition of clomiphene citrate (CC) to human menopausal gonadotropin (hMG) during intrauterine insemination (IUI) cycles to prevent a premature luteinizing hormone (LH) surge. Their study was well designed. Surprisingly, the endometrial thickness of both groups w thin, with an average thickness of less than 6.0 mm, which may reduce pregnancy rates as the authors mentioned. Read the rest of this entry »





Inositol effects on ovulation induction in patients with polycystic ovary syndrome.

28 03 2011

To the Editor:

I read with interest the recent paper of Morgante et al (1) describing positive effects of Inositol in increasing insulin sensitivity in patients with polycystic ovary syndrome (2), confirming the concept that insulin-sensitizer molecules alone or in combination with clomiphene citrate or gonadotrophin, somehow ameliorates ovulation in such patients.

However some comments on both design and results of the study are necessary for a critical view of potential clinical application of inositol in ART. Read the rest of this entry »





An explanation for the true origin of spontaneous pregnancies after subcutaneous ovarian transplantation

28 03 2011

To the Editor:

I read with interest the article by Oktay et al. (1), in which the authors reported spontaneous pregnancies and live births after subcutaneous heterotopic ovarian transplantation to a Hodgkin lymphoma survivor who was rendered menopausal because of preconditioning chemotherapy before hematologic stem cell transplantation (HSCT), and discussed the origin of pregnancies after ovarian transplantation. They have done a good work.

I have great interest in the true origin of the spontaneous pregnancies. Although they mentioned that this patient might get pregnant without heterotopic transplantation, they did believe other explanations need more laboratory and clinical research because the third pregnancy occurred after the patient did not respond to high doses of gonadotropins and her in situ ovary was invisible by ultrasound examination. Read the rest of this entry »





Fertility in patients with 5 alpha-reductase-2 deficiency

23 03 2011

To the Editor:

The case report by Matsubara et al. (1) is very interesting and an extensive study for the diagnosis of 5 alpha-reductase-2 deficiency.

The authors reported the successful birth of a healthy male child through intracytoplasmic sperm injection (ICSI). It is imperative to test the genotype of the newborn, since there was a strong familial aggregation (both grandparents were heterozygous, father was homozygous for p.R246Q mutation on exon 5) of SRD5A2 gene mutations in this family. Read the rest of this entry »





Vitrification with UV-sterilized super-cooled air

22 03 2011

To the Editor:

We read with interest the recent article by Larman and Gardner (1) regarding their alternative technique for vitrifying oocytes/embryos with the carrier Rapid-i. The authors proposed inserting the Rapid-i into the super-cooled air of a straw for instantaneous vitrification and then sealing the open end of the straw (postsealing method) in order to avoid direct contact with liquid nitrogen (LN2). We would like to point out that the super-cooled air inside the straws will be basically composed of nitrogen vapors, due to the nitrogen’s rapid evaporation and molecular weight. For this reason any microorganism accidentally present in the LN2 can also pass to the nitrogen vapor phase (2), and this may lead to the hypothetical contamination of the oocyte/embryos and the inner carrier of Rapid-I. Read the rest of this entry »