Proficiency in oocyte retrieval: plausible steps before perfection

22 08 2011

To the Editor:

We read with interest the retrospective analysis by Goldman et al (1). The authors attempt to determine the minimum number of procedures required for proficiency in oocyte retrieval (OR). Assessing and classifying the technical competence of a trainee objectively is quite difficult for two reasons: first, there is no clear definition of “competence,” as operative skill is a combination of knowledge, judgment, and technical ability (2). Second, the success of an OR depends on additional parameters, such as patient, nurse and embryologist compliance. We would like to share our thoughts on their work. Read the rest of this entry »

Melatonin protects human spermatozoa from apoptosis via melatonin receptor– and extracellular signal–regulated kinase-mediated pathways

8 08 2011

To the Editor:

The paper of Espino et al. (1) addressed a very interesting issue about the anti-apoptotic activity of the pineal hormone melatonin in human semen. Employing 1 mM doses of melatonin in vitro and the MT1/MT2 receptor antagonists luzindole and 4-phenyl-2-propionamidotetralin (4P-PDOT), the authors claim to have demonstrated that “…melatonin prevention of H2O2-induced DNA fragmentation is dependent on …MT1 receptor ….”. We find that above statement may be arguable and there is need of further exploration and clarification of the mechanisms involved. Read the rest of this entry »