To the Editor:
We read with great interest the article by Avendaño et al (1), “Use of laptop computers connected to internet through Wi-Fi decreases human sperm motility and increases sperm DNA fragmentation.” We want to congratulate the authors for their original and interesting work. However, this study has also raised a few questions.
First of all, the issue of sperm analysis method should be addressed. Indeed, sperm analysis was performed manually before selection, after swim up, and after 4 hours of incubation under the computer or not (control group). Despite efforts towards improvement and standardization of laboratory procedures (2), manual sperm analysis still suffers from a lack of precision and accuracy (3), particularly when counting progressive motile spermatozoa in an enriched supernatant. Moreover, sperm analysis was conducted here by a single observer and apparently not repeated, which leads to a high risk of intra observer variability. We also would like the authors to precise if the study was operator-blinded or if the operator was aware of the origin of the samples he was assessing. All these limitations could constitute a bias and prevent from relevant interpretation of the results. Could the authors consider automated sperm analysis as a reliable alternative in order to improve reproducibility and precision of their results? (4) Moreover, CASA could bring new insights into a potential WiFi-induced sperm motility alteration and kinetics modification. Read the rest of this entry »