Vitrification Carriers and European Regulation

6 03 2012

To the Editor:

We read with interest the recent article by Valbuena et al. (1) comparing the efficiency of Cryotip vs. Cryotop for blastomere vitrification. The authors concluded that it is preferable to preserve individual human blastomeres using Cryotip, which is a closed system. Furthermore, they suggest that this has the advantage of complying with European Union directives.

We would like to point out that European Union directives on tissue manipulation (European Union Tissues and Cells Directive EUTCD: 2004/23/EC, 2006/17/EC and 2006/86/EC) have been issued by the European Parliament in order to increase the safety and quality of tissues – including reproductive cells – processed for human re-implantation through the control of equipment, devices and environment. These regulations require specific procedures in embryo/oocyte/ovarian tissue cryopreservation in order to minimize the risk of any hypothetical contamination of human cells due to direct contact with accidentally contaminated liquid nitrogen (LN2). Read the rest of this entry »


Contamination of single-straw carrier for vitrification

11 04 2011

To the Editor:

We read with interest the recent article by Criado et al. (1) regarding the assessment of contamination of a single-straw ultravitrification closed carrier (Ultravit). They observed no contamination in any microdrops of medium contained in the closed devices, whereas the bacteria they used for forced contamination of liquid nitrogen (LN2) were present in 45% of open carriers’ strip (Cryotop). Read the rest of this entry »